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Main Title Highly sensitive bioassays for evaluating airborne mutagens indoors. {microfiche} / by S. Goto, et al.
Author Goto, S. ; Takagi, Y. ; Endo, O. ; Lewtas, J. ; Matsushita., H.
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;National Inst. of Public Health, Tokyo (Japan). ;Azabu Univ., Sagamihara (Japan).
Publisher US Environmental Protection Agency, Health Effects Research Laboratory
Year Published 1991
Report Number EPA/600/D-90/165
Stock Number PB91-132985
Additional Subjects Mutagens ; Mutations ; Bioassay ; Salmonella typhimurium ; Smoke ; Tobacco ; Foreign technology ; Indoor air pollution ; Mutagenicity tests ; Metabolic activation
Library Call Number Additional Info Location Last
NTIS  PB91-132985 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9 p.
The standard mutagenicity bioassays that are readily applied to the evaluation of outdoor air samples collected by high volume samplers are not sufficiently sensitive to measure the mutagenicity of low volume air samples collected indoors. Two microsuspension mutation assays using various strains of Salmonella typhimurium were evaluated for the assessment of indoor air pollution. One assay used highly sensitive YG strains of bacteria in a histidine reversion assay. The other was a forward mutation assay detecting 8-azaguanine resistant mutants in the TM677 strain. To decrease the required sample mass in the forward mutation assay, the volume in the preincubation step was decreased 10-fold to produce an ultra microsuspension assay. By applying these assays to indoor air samples, their usefulness was confirmed in investigating the effect of smoking, ventilation or air conditioning.