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Main Title Application of the Micro-Forward Mutation Assay to Assess Mutagenicity of Airborne Particulates in Indoor.
Author Takagi, Y. ; Goto, S. ; Murata, M. ; Matsushita, H. ; Lewtas., J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/542;
Stock Number PB91-109231
Additional Subjects Air pollution ; Toxicity ; Mutations ; Particle size ; Salmonella typhimurium ; Benzene ; Ethyl alcohol ; Foreign technology ; Mutagenicity test ; Indoor air pollution ; Metabolic activation
Library Call Number Additional Info Location Last
NTIS  PB91-109231 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
A validity test of the micro-forward mutation assay using Salmonella typhimurium strain TM677 was carried out using benzene-ethanol extracts from airborne particulates as test materials. Sensitivity of this assay in the presence and absence of S-9 mix was five to ten times higher than the pre-incubation method, a modified method of Ames's original method, using Salmonella typhimurium strains TA98 and TA100. This means that the virtual sensitivity of the micro-forward mutation assay is greater by ten times or more than the pre-incubation method, because two or more strains are necessary in the latter method. Repeatability of the micro-forward mutation assay was nearly same with that of the pre-incubation method. That is, the coefficient of variation of mutagenic frequency of the particulate extracts was about 10%. This forward mutation assay was applied to airborne particulates indoors, and proved to be able to measure easily the mutagenic activity of the extracts from particulates collected by 30 cu m-air sampling. Some indoor pollutants, especially ones collected in a room in which cigarette smoking was done, showed higher mutagenic activity than that of outdoor sample.