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Main Title 2,4,5-Trichlorophenoxyacetic Acid Influence on 2,6-Dinitrotoluene-Induced Urine Genotoxicity in Fisher 344 Rats: Effect on Gastrointestinal Microflora and Enzyme Activity.
Author George, S. E. ; Chadwick, R. W. ; Chang, J. J. ; Kohan, M. J. ; Allison, J. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;North Carolina Univ. at Chapel Hill. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/250;
Stock Number PB92-206309
Additional Subjects Toxicity ; Mutagens ; Microbiology ; Gastrointestinal system ; Enzymes ; Rats ; Urine ; Dose-response relationships ; Metabolism ; Metabolic activation ; Reprints ; Trichlorophenoxyacetic acids ; Dinitrotoluenes
Library Call Number Additional Info Location Last
NTIS  PB92-206309 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
2,4,5-Trichlorophenoxyacetic acid and 2,6-dinitrotoluene are hazardous chemicals that have potential harmful effects. 2,6-DNT is recognized as a hepatoxicant while 2,4,5-T, a component in Agent Orange, is also suspect. 2,6-DNT requires both oxidative and reductive metabolism to elicit genotoxic effects. In order to determine what effect 2,4,5-T had on 2,6-DNT metabolism, intestinal enzymes, microbial populations, and urine mutagenicity were examined during 2,4,5-T treatment. Weanling Fischer 344 male rats were treated daily with 54.4 mg/kg 2,4,5-T by gavage for 4 weeks. 1,2, and 4 weeks after the initial dose, rats were administered (p.o.) 2,6-DNT (75 mg/kg) and urine was collected for 24 hours in metabolism cages. Azo reductase, nitroreductase, beta-glucuronidase, dechlorinase, and dehydrochlorinase activities were examined concurrently. Treatment of rats for one week reduced the transformation of 2,6-DNT to mutagenic urinary metabolites. This was accompanied by a decrease in the fecal anaerobic microorganisms. The elimination of Lactobacillus fermentum from the small intestine and cecum of treated animals accompanied a significant increase in oxygen tolerant lactobacilli and other unidentified aerobic microorganisms. (Copyright (c) 1992 by the Society of Toxicology.)