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Main Title (32)P-Postlabeling Analysis of DNA Adducts of Two Nitrated Polycyclic Aromatic Hydrocarbons in Rabbit Tracheal Epithelial Cells.
Author Gallagher, J. E. ; Robertson, I. G. C. ; Jackson, M. A. ; Dietrich, A. M. ; Ball, L. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.
Year Published 1987
Report Number EPA/600/D-87/240;
Stock Number PB88-103718
Additional Subjects Aromatic polycyclic hydrocarbons ; Deoxyribonucleic acids ; Cells(Biology) ; Epithelium ; Trachea ; Rabbits ; Marking ;
Library Call Number Additional Info Location Last
NTIS  PB88-103718 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The 1-nitropyrene (1-NPP and 3-nitrofluoranthene (3-NF) adducts have been analyzed by (32)P-postlabeling and with 1-NP have been compared to the total number of adducts estimated from (14)C binding in rabbit trachael epithelial (RTE) DNA samples. One adduct spot, by (32)P-postlabeling, was detected in the DNA of RTE cells treated with (14)C-1-NP. The adduct, at approximately 6 adducts 10-to the 9th power nucleotides, represents a minor component of the binding to RTE DNA being lesser than 1% of the total (14)C-radioactivity associated with the extracted DNA. Treatment of RTE cells with unlabeled 3-NF resulted in two adduct spots at approximately 12 adducts/10 to the 9th power. When the P1 nuclease treatment was used as an alternative method both the 3-NF derived adducts previously detected following the butanol extraction were absent.