Record Display for the EPA National Library Catalog


Main Title Dose-Related Differences in DNA Adduct Levels in Rodent Tissues Following Skin Application of Complex Mixtures from Air Pollution Sources.
Author Gallagher, J. E. ; Jackson, M. A. ; George, M. H. ; Lewtas, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/037;
Stock Number PB90-217571
Additional Subjects Toxicity ; Skin(Anatomy) ; Carcinogens ; Mice ; Tables(Data) ; Graphs(Charts) ; Aromatic polycyclic hydrocarbons ; Reprints ; Dose-reresponse relationships ; Air pollution effects(Animals) ; DNA damage ; Autoradiography
Library Call Number Additional Info Location Last
NTIS  PB90-217571 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Dose-related differences in the binding of DNA reactive intermediates for three environmentally important complex particulate extracts and a well studied carcinogen Benzo(a)Pyrene (B(a)P) were examined in female C-57 mice following multiple topical treatments ranging from (1-120) mg per mouse. Particulate extracts from coke oven, coal soot and diesel exhaust were selected as model complex mixtures based on short term mutagenicity assays, animal bioassays for carcinogenicity or epidemiological studies where increased incidences of lung cancer in exposed populations were detected. Positive and negative control animals were treated with either 1.2 mg Benzo(a)Pyrene or acetone alone. DNA was isolated from skin, lung and liver DNA 24 hours following the last application and analyzed for DNA adducts using the nuclease P1 version of the 32p-postlabeling assay. Each of the particulate extracts produced distinct patterns of DNA adducts. A broad diagonal radioactive zone (DRZ) presumably representing multiple DNA adducts, was observed for coke oven, coal soot and diesel modified DNA samples. One adduct, common to all three complex mixture modified DNA samples, comigrated with the major B(a)P adduct observed following treatment with B(a)P alone. These data have important implications for low dose risk assessment of these complex mixtures. (Copyright (c) Oxford University Press.)