Record Display for the EPA National Library Catalog


Main Title Relative Sensitivity of (32)P-postlabelling of DNA and the Autoradiographic UDS Assay in the Liver of Mice Exposed to 2-acetylaminofluorene (2AAF).
Author Ashby, J. ; Lefevre, P. A. ; Shank, T. ; Lewtas, J. ; Gallagher., J. E. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Imperial Chemical Industries Ltd., Alderley Park (England). Central Toxicology Lab.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/162;
Stock Number PB91-231308
Additional Subjects 2-Acetylaminofluorene ; Deoxyribonucleic acids ; DNA repair ; Liver ; Toxicity ; Autoradiography ; Phosphorus 32 ; Dose-response relationships ; Mice ; Carcinogens ; Metabolism ; In vivo analysis ; DNA damage ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-231308 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
In contrast to earlier studies conducted at lower dose levels, 2AAF is shown to induce a positive UDS response in the liver of mice dosed orally at dose levels between 500 and 1000 mg/kg. Similarly exposed mice had low levels of 2AAF-related hepatic DNA adducts at dose levels in the range 10-1000 mg/kg 2AAF, as determined by (32)P-postlabelling analysis. It is concluded that the attenuated UDS response observed in the mouse liver, as compared to the rat liver, is due primarily to metabolic differences between these two species, coupled to a reduced capacity for UDS in the mouse liver for a given level of total 2AAF-related adducts per unit of DNA. (Copyright (c) 1991 Elsevier Science Publishers B.V.)