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Main Title Phenol and Trichloroethylene Degradation by 'Pseudomonas cepacia' G4: Kinetics and Interactions between Substrates.
Author Folsom, B. R. ; Chapman, P. J. ; Pritchard, P. H. ;
CORP Author Technical Resources, Inc., Gulf Breeze, FL.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA-R-68033479; EPA/600/J-90/110;
Stock Number PB90-264201
Additional Subjects Vinyl chloride ; Phenol ; Biodeterioration ; Kinetics ; Enzymes ; Reprints ; Pseudomonas cepacia ; Substrate specificity
Library Call Number Additional Info Location Last
NTIS  PB90-264201 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Intact cells of Pseudomonas cepacia strain G4 completely degraded trichloroethylene (TCE) following growth with phenol. Degradation kinetics were determined for both phenol, used to induce requisite enzymes, and TCE, the target substrate. At phenol concentrations above 50 microM, phenol degradation was inhibited, yielding an apparent K(sub i) of 0.45 mM as modeled by the Haldane expression. To eliminate experimental problems associated with TCE volatility and partitioning, a 'no-headspace' assay was developed allowing for direct and accurate determinations of aqueous TCE concentration. Using this assay procedure, apparent K(s) and V(max) values determined for TCE degradation by intact cells were 5.6 microM and 7.9 nmole/(min times mg protein) respectively. Following a transient lag period, P. cepacia G4 degraded TCE at concentrations of at least 300 microM TCE with no apparent retardation in rate.