Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Effects of Physicochemical Factors and Bacterial Colony Morphotype on Association of 'Vibrio vulnificus' with Hemocytes of 'Crassostrea virginica'.
Author Harris-Young, L. ; Tamplin, M. L. ; Fisher, W. S. ; Mason, J. W. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Alabama Univ. in Birmingham. School of Public Health. ;Food and Drug Administration, Dauphin Island, AL. Fishery Research Branch. ;Florida Univ., Gainesville.;National Oceanic and Atmospheric Administration, Rockville, MD.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-94/012;
Stock Number PB94-140688
Additional Subjects Bacteria ; Diseases ; Humans ; Oysters ; Food consumption ; Tolerances(Physiology) ; Temperature ; Concentration(Composition) ; Serum ; Tissues(Biology) ; Estuaries ; Reprints ; Crassostrea virginica ; Hemocytes
Library Call Number Additional Info Location Last
NTIS  PB94-140688 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 05/14/1994
Collation 8p
Vibrio vulnificus is a naturally occurring marine bacterium which causes invasive disease of immunocompromised humans following consumption of raw oysters. It is natural flora of Gulf Coast estuaries and has been found to inhabit tissues of oysters, Crassostrea virginica (Gmelin, 1791). The interaction of V. vulnificus with oyster host defenses has not been reported in detail. In the present studies, we examined the interaction of V. vulnificus with phagocytic oyster hemocytes in response to time, temperature, bacterial concentration, pretreatment in hemolymph, and with V. vulnificus translucent and opaque colonial morphotypes. Within these experimental parameters, results showed that association of V. vulnificus with hemocytes increased with time, temperature, and V. vulnificus:hemocyte ratio. Pretreatment of V. vulnificus with serum, or increased serum concentration, did not enhance V. vulnificus-hemocyte associations, indicating undetectable opsonic activity. More than 50% of hemocytes bound the translucent, avirulent morphotype, whereas 10% to 20% were associated with the opaque, virulent form. (Copyright (c) 1993, American Society for Microbiology.)