Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 5

Main Title Multilaboratory Evaluation of Methods for Detecting Enteric Viruses in Soils.
Author Hurst, C. J. ; Schaub, S. A. ; Sobsey, M. D. ; Farrah, S. R. ; Gerba, C. P. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. ;Florida Univ., Gainesville. Dept. of Microbiology and Cell Science. ;Arizona Univ., Tucson. Dept. of Microbiology and Immunology.;Army Biomedical Research and Development Lab., Fort Detrick, MD.
Publisher cFeb 91
Year Published 1991
Report Number EPA/600/J-91/013;
Stock Number PB91-183533
Additional Subjects Enteroviruses ; Soil microbiology ; pH ; Statistical analysis ; Soil chemistry ; Wastewater ; Water microbiology ; Reprints ;
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NTIS  PB91-183533 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Abstract
Two candidate methods for the recovery and detection of viruses in soil were subjected to round robin comparative testing by members of the American Society for Testing and Materials D19:24:04:04 Subcommittee Task Group. Selection of the methods was based upon results of an initial screening which indicated that both met basic criteria considered essential by the task group. Both methods utilized beef extract solutions to achieve desorption and recovery of viruses from representative soils: a fine sand soil; an organic muck soil; a sandy loam soil; and a clay soil. The Berg method seemed to produce slightly higher virus recovery values, however the differences in virus assay titers for samples produced by the two methods were not statistically significant (p < or = 0.05) for any one of the four soils. Despite this lack of a Method Effect, there was a statistically significant Laboratory Effect exhibited by assay titers from the independent versus reference laboratories for two of the soils, the sandy loam and clay.