Record Display for the EPA National Library Catalog


Main Title Confirmed Viruses Versus Unconfirmed Plaques in Sewage.
Author Bertucci, J. J. ; Abid, S. H. ; Lue-Hing, C. ; Clark, C. S. ; Fenters, J. D. ;
CORP Author Metropolitan Sanitary District of Greater Chicago, IL.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA-R-803643 ;EPA-R-805003; EPA-600/J-83-054;
Stock Number PB84-101328
Additional Subjects Viruses ; Sewage ; Cell cultures ; Etiology ; Assaying ; Reprints ; Plaques ; Cell cultures
Library Call Number Additional Info Location Last
NTIS  PB84-101328 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 19p
Ninety-two treated and untreated sewage samples from seven wastewater treatment plants in Chicago, Illinois, Memphis, Tennessee, and Cincinnati, Ohio were examined for their virus content. Concentrated and unconcentrated samples were plaque assayed in five different cell culture systems. The virus etiology of plaques was confirmed by passage in homologous cell fluid cultures. Including all plaques tested, only 16.5 percent were confirmed as of viral origin. BGM cultures produced the greatest number of unconfirmable plaques. Confirmed virus concentrations for individual samples ranged from less than 0.2 to 80 PFU/1. Confirmed PFU/1 averaged 0.7 for raw sewage, 0.5 for preliminary effluents and were not detected in final effluents. A strong recommendation of this study is that the virus etiology of all environmentally derived plaques must be confirmed.