Record Display for the EPA National Library Catalog

RECORD NUMBER: 2 OF 4

OLS Field Name OLS Field Data
Main Title Sequence Similarities in the Genes Encoding Polychlorinated Biphenyl Degradation by 'Pseudomonas' Strain LB400 and 'Alcaligenes eutrophus' H850.
Author Yates, J. R. ; Mondello, F. J. ;
CORP Author General Electric Co., Schenectady, NY. Research and Development Center.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-93/275;
Stock Number PB93-229201
Additional Subjects Bacteria ; Biodegradation ; Polychlorinated biphenyls ; Bacterial genes ; DNA hybridization ; Oxidation ; Cell constitutents ; Genetic engineering ; Genetics ; Microbiology ; Biochemistry ; Reprints ; Pseudomonas strain LB400 ; Alcaligenes eutrophus H850 ; Congeners ; Strains(Biology) ; Gene sequences
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB93-229201 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 11/22/1993
Collation 5p
Abstract
DNA-DNA hybridization was used to compare the Pseudomonas strain LB400 genes for polychlorinated biphenyl (PCB) degradation with those from seven other PCB-degrading strains. Significant hybridization was detected to the genome of Alcaligenes eutrophus H850, a strain similar to LB400 in PCB-degrading capability. These two organisms showed a strong conservation of restriction sites in the region of DNA encoding PCB metabolism. No other sequence similarities were detected in the two genomes. DNA from the other PCB-degrading strains showed no hybridization to the probe, which demonstrated the existence of at least two distinct classes of genes encoding PCB degradation. (Copyright (c) 1989, American Society for Microbiology.)