Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Teratogen Metabolism: Thalidomide Activation is Mediated by Cytochrome P-450.
Author Braun, A. G. ; Harding, F. A. ; Weinreb, S. L. ;
CORP Author Massachusetts Inst. of Tech., Cambridge. Dept. of Applied Biological Sciences.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA/600/J-86/089;
Stock Number PB86-208527
Additional Subjects Toxicology ; Metabolism ; Reprints ; Thalidomide ; Metabolites ; Teratogenesis
Library Call Number Additional Info Location Last
NTIS  PB86-208527 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 7p
A metabolite of thalidomide generated by hepatic microsomes inhibited the attachment of tumor cells to concanavalin A-coated polyethylene. Evidence that metabolite formation is mediated by microsomal cytochrome P-450 is presented. Microsomes incubated with thalidomide underwent a type I spectral shift. Metabolite formation was reduced or eliminated by carbon monoxide, SKF-525A, metyrapone, and N-octylamine. Superoxide dismutase treatment had no effect. Metabolite formation required microsomes and NADPH and was dependent on the length of 37 C incubation. The metabolite could be isolated by successive hexane and chloroform extractions. It is likely the inhibitory thalidomide metabolite was generated by a minor cytochrome P-450 species. Whether the thalidomide metabolite is involved in the drug's teratogenic activity remains to be shown.