Record Display for the EPA National Library Catalog


Main Title Genetic Bioassay Branch Protocol for Bacterial Mutagenesis Including Safety and Quality Assurance Procedures.
Author Claxton, Larry D. ; Kohan, Michael ; Austin, Ann C. ; Evans, Carol ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1982
Report Number EPA-600/2-82-008;
Stock Number PB82-220831
Additional Subjects Manuals ; Bacteria ; Tests ; Bioassay ; Safety ; Procedures ; Genetics ; Microorganisms ; Mutagenesis ; Protocols ; Quality assurance
Library Call Number Additional Info Location Last
NTIS  PB82-220831 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 159p
Researchers often choose microbial tests for use in genetic toxicology studies because they are rapid and inexpensive, and because they supply dose-responsive data. This user's manual documents the test methods used extensively by the Health Effects Research Laboratory (HERL), Research Triangle Park, North Carolina over the past four years. The first part of the report describes the basic protocol (the plate incorporation test), special precautions, and specific modifications of the assay routinely used in HERL studies. The second part presents HERL's stepwise protocol for the plate incorporation test and protocols for several other biological assays, including the spot test, the forward mutation assay procedure, and the fluctuation test. The last portion of the report provides the necessary information for experiment planning and provides sample information forms and computerization procedures necessary for reporting experimental results.