Record Display for the EPA National Library Catalog


Main Title Flow Cytometric Analysis of the Cellular Toxicity of Tributyltin.
Author Zucker, R. M. ; Elstein, K. H. ; Easterling, R. E. ; Massaro, E. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc./Environmental Sciences, Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/489;
Stock Number PB90-186263
Additional Subjects Toxicity ; Deoxyribonucleic acids ; Cytoplasm ; Proteins ; Cells(Biology) ; Light scattering ; Reprints ; Tributylin ; Flow cytometry ; Fluorescence microscopy ; Dose-response relationships
Library Call Number Additional Info Location Last
NTIS  PB90-186263 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 20p
Flow cytometric and light/fluorescence microscopic analyses indicate that tributylin (TBT) alters the plasma membrane/cytoplasm complex of the murine erythroleukemic cell (MELC) in a dose-dependent and time-dependent manner. The flow cytometric parameter axial light loss, a measure of cell volume, decrease cell exposed to 5 mu M TBT relative to control cells or cells exposed to 50 mu M TBT. The flow cytometric parameter 90 degree light scatter, a function of refractive index and a measure of protein content, increases as a function of TBT concentration above 0.5 mu M. Following exposure to TBT concentrations greater than 0.5 mu M but less than 50 mu M, DNA distribution across the cell cycle cannot be resolved adequately by flow cytometry. Also, the cells become resistant to solubilization of the cell membrane/cytoplasm complex by nonionic detergents.