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Main Title Flow Cytometric Analysis of the Cellular Toxicity of Tributyltin, September 1989.
Author Zucker, R. M. ; Elstein, K. H. ; Easterling, R. E. ; Massaro, E. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. ;Harry G. Armstrong Aerospace Medical Research Lab., Wright-Patterson AFB, OH.
Publisher Sep 89
Year Published 1989
Report Number EPA/600/D-90/043;
Stock Number PB90-220773
Additional Subjects Proceedings ; Toxicology ; Hydrolysis ; Deoxyribonucleic acids ; Tributyltin ; Membrane potential ; Cell survival ; Flow cytometry ; Fluorescence microscopy ; Cultured tumor cells ; Cell membrane permeability
Library Call Number Additional Info Location Last
NTIS  PB90-220773 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 22p
Flow cytometric and light/fluorescence microscopic analyses indicate that tributyltin (TBT) alters the plasma membrane/cytoplasm complex of the murine erythroleukemic cell (MELC) in a dose dependent and time-dependent manner. The flow cytometric parameter axial light loss, a measure of cell volume, decreases in cells exposed to 5 microM TBT relative to control cells or cells exposed to 50 microM TBT. The flow cytometric parameter 90 deg light scatter, a function of refractive index and a measure of protein content, increases as a function of TBT concentration above 0.5 microM, but less than 50 microM DNA distribution across the cell cycle cannot be resolved adequately by flow cytometry. Also, the cells become resistant to solubilization of the cell membrane/cytoplasm complex by nonionic detergents. Relative to logarithmically growing cells, MELC in the stationary phase of the growth cycle and butyric acid-differentiated cells exhibit decreased plasma membrane permeability resulting in increased carboxyfluorescein (CF) retention derived from the intracellular hydrolysis of carboxyfluorescein diacetate (CFDA). Similarly, cells exposed to TBT concentrations below 50 microM exhibit increased cellular CF retention. Viability in terms of CFDA hydrolysis/CF retention and propidium iodide (PI) exclusion is not decreased by exposure to TBT concentrations below 1 microM. At doses between 5 and 50 microM, however, cells exhibit both CF and PI fluorescence simultaneously and are programmed for death.