Record Display for the EPA National Library Catalog


Main Title Metal-Induced Alteration of the Cell Membrane/Cytoplasm Complex Studied by Flow Cytometry and Detergent Lysis.
Author Zucker, R. M. ; Elstein, K. H. ; Easterling, R. E. ; Massaro, E. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;NSI Technology Services Corp., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/487;
Stock Number PB90-185190
Additional Subjects Toxicology ; Cadmium ; Zinc ; Cytoplasm ; Detergents ; Fluorescence ; Cell nucleus ; Reprints ; Tributyltin ; Cell membrane ; Cultured tumor cells ; Flow cytometry
Library Call Number Additional Info Location Last
NTIS  PB90-185190 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Flow cytometric analysis of the cell cycle is most effectively accomplished with membrane-/cytoplasma-free (clean) nuclei. Non-ionic detergents (e.g. NP40 or Triton X-100) commonly are employed to solubilize cells membranes/cytoplasm to produce 'clean' nuclei. Treatment of murine erythroleukemic cells with tri-n-butylin methoxide, cadmium acetate, zinc sulfate, or lead acetate alters the properties of the cell membrane/cytoplasm complex making it resistant to NP40 dissolution. On a molar basis, the organotin compound was more effective in inducing resistance to detergent-mediated dissolution than the inorganic metal compounds. Resistance to NO40-mediated dissolution was manifested as an increase in the flow cytometric parameters 90 degree scatter and fluorescein isothiocyanate fluorescence and was confirmed by light microscopy. (Copyright (c) Elsevier Scientific Publishers Ireland, Ltd.)