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Main Title Formation and Persistence of DNA Adducts in the Liver of Brown Bullheads Exposed to Benzo(a)Pyrene.
Author Sikka, H. C. ; Rutkowski, J. P. ; Kandaswami, C. ; Kumar, S. ; Earley., K. ;
CORP Author State Univ. of New York Coll. at Buffalo. Great Lakes Lab. ;Baylor Coll. of Medicine, Houston, TX.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA-R-813840; EPA/600/J-90/087;
Stock Number PB90-245390
Additional Subjects Fresh water fishes ; Toxicology ; Liver ; Tissues(Biology) ; Liquid chromatography ; Reprints ; DNA damage ; Benzo(a)pyrene ; Water pollution effects(Animals) ; Mutagenicity tests ; Binding sites ; Metabolic activation ; Carcinogenicity tests ; Autoradiography ; Ictalurus nebulosus
Library Call Number Additional Info Location Last
NTIS  PB90-245390 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The formation and persistence of benzo(a)pyrene (BP)-DNA adducts in the liver of brown bullheads (Ictalurus nebulosus) treated with the hydrocarbon (20 mg/kg body wt, i.p.) was investigated using the (sup 32)P-postlabeling assay. The highest level of covalent binding of BP to liver DNA (188 fmol BP adducts/mg DNA) was observed 25-30 days following treatment. After 70 days, the adduct level in liver DNA had declined to approximately 26% of the maximum adduct level. The data suggest that brown bullheads metabolically activate BP by the same mechanism as the mammalian systems susceptible to carcinogenic effects of the hydrocarbon. (Copyright (c) 1990 Elsevier Scientific Publishers Ireland Ltd.)