Record Display for the EPA National Library Catalog


Main Title Extraction of DNA from Cryopreserved Clotted Human Blood.
Author Everson, R. B. ; Mass, M. J. ; Gallagher, J. E. ; Musser, C. ; Dalzell, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Carcinogenesis and Metabolism Branch. ;SRA Technologies, Inc., Research Triangle Park, NC.
Publisher 1993
Year Published 1993
Report Number EPA/600/J-94/027;
Stock Number PB94-137270
Additional Subjects Deoxyribonucleic acids ; Blood ; Biological preservation ; Freezing ; Hemoglobins ; Blood coagulation ; Epidemiology ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-137270 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
Clinical and epidemiologic investigations into the etiology of cancer and other chronic diseases are increasingly reliant on laboratory analyses to characterize exposures, susceptibilities, and biological effects in human populations. Obtaining these data for sizeable human populations will require access to large numbers of specimens of human body fluids or tissues. DNA or hemoglobin are commonly needed for the laboratory analyses. For some studies the only available specimens are samples of clotted blood. For example, the Third National Health and Nutrition Examination Survey III (NHANES III) will obtain extensive questionnaire and laboratory data from a stratified multistage probability sample of 15,000 subjects of the U.S. population, including clotted blood from a 70 ml drawn from each adult. This paper presents a method for obtaining DNA and hemoglobin from these whole blood clots. It involves application of widely used methods for red cell lysis, protein digestion, and phenol/water/chloroform DNA extraction. Application of the procedure to specimens currently being collected and discarded by studies such as the NHANES III will provide a unique and cost effective research opportunity to help define the magnitude of human exposure to certain environmental pollutants and investigate susceptibility factors in a large probability sample of U.S. residents, information that could play a critical role in both assessing risk and determining health effects from environmental exposures.