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Main Title Biosynthesis of Dimethylnitrosamine in Dimethylamine-Treated Mice After Exposure to Nitrogen Dioxide.
Author Iqbal, Zafar M. ; Dahl, Kristine ; Epstein, Samuel S. ;
CORP Author Illinois Univ. at the Medical Center, Chicago.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-R-807293; EPA-600/J-81-609;
Stock Number PB83-169706
Additional Subjects Nitrogen dioxide ; Biosynthesis ; Ingestion(Biology) ; Mice ; Exposure ; Laboratory animals ; Dimethylamine ; Nitrogen organic compounds ; Reprints ; Dimethylamine/N-nitroso
Library Call Number Additional Info Location Last
NTIS  PB83-169706 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
These studies demonstrate the nitrosating potential of NO2 in vivo in 1CR mice. Groups of mice were gavaged with 2 mg dimethylamine (DMA) and exposed to NO2 at levels from 0.04 to 44.5 ppm for periods up to 4 hours. Mice were individually frozen and blended to a powder, aliquots of which were homogenized in ice-cold dichloromethane and 35% aqueous methanol. Concentrates of organic extracts were analyzed for dimethylnitrosamine (DMN) by a Thermal Energy Analyzer with a gas chromatograph interface. Biosynthesis of DMN was dose- and time-dependent with relation to NO2 exposure, reaching a maximum yield of 60-70 ng/mouse (0.0034% DMA conversion) at 2 hours. DMN biosynthesis was inhibited by sodium ascorbate and, more effectively, by ammonium sulfamate.