||Bioassay Protocol for Lethal and Sub-Lethal Effects of Fungal Pathogens on 'Chrysoperla carnea' (Neuroptera: Chrysopidae).
Donegan, K. ;
Lighthart, B. ;
||Corvallis Environmental Research Lab., OR. ;ManTech Environmental Technology, Inc., Corvallis, OR.
Biological pest control ;
Quality control ;
Lethal dosage ;
Chrysoperla carnea ;
||Some EPA libraries have a fiche copy filed under the call number shown.
The protocol describes procedures for evaluating the lethal and sublethal effects of exposure to fungal pathogens on larvae and adults of the predatory insect Chrysoperla carnea (Stephens). The protocol was developed and tested with the fungal insect pathogen Beauveria bassiana (Balsamo) under environmental conditions selected to maximize insect susceptibility to the pathogen. The procedures have not been performed with viral or protozoan preparations and only in part with bacterial pathogens. Successful execution of the procedures will require training in microbiological techniques, arthropod rearing and elementary statistical analysis.