Record Display for the EPA National Library Catalog


Main Title Mutagenicity of Actinomycin D in Mammalian Cells Due to Clastogenic Effects.
Author DeMarini, D. M. ; Brock, K. H. ; Doerr, C. L. ; Moore, M. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/J-87/244;
Stock Number PB88-177142
Additional Subjects Cells(Biology) ; Mammals ; Reprints ; Mutagenesis ; Actinomycin D ; Clastogenesis
Library Call Number Additional Info Location Last
NTIS  PB88-177142 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Actinomycin D was clastogenic and mutagenic in L5178Y/TK+/- -3.7.2C mouse lymphoma cells. The majority of the mutants were small colonies, indicating that actinomycin D acts primarily by a clastogenic mechanism. The authors have been studying the mutagenicity of a series of agents that are highly clastogenic and induce DNA breaks but that are not mutagenic (or give very low mutant frequencies) in prokyrotic systems and at functionally heterozygous tk locus and prt) in mammalian cells. They have been evaluating agents such as actinoycin D at the functionally heterozygous tk locus in L5178Y/TK+/- -3.7.2.C mouse lymphoma cells to determine if these agents, which induce low mutant frequencies at the hemizygous hprt locus, might induce high mutant frequencies at the heterozygous tk locus. L5178Y/TK+/- -3.7.2.C mouse lymphoma cells permit the measurement of forward mutation at the functionally heterozygous tk locus, and extensive studies indicate that these cells may detect both single-gene (intragenic) mutations as well as chromosomal mutations (intragenic events resulting from chromosomal rearrangements and/or functional multilocus deletions) affecting the expression of the tk locus.