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Main Title Study of the Binding of Sulfur to Rat Liver Microsomes which Occurs Concurrently with the Metabolism of O,O-Diethyl O-p-Nitrophenyl Phosphorothioate (Parathion) to O,O-Diethyl O-p-Nitrophenyl Phosphate (Paraoxon).
Author Davis, James E. ; Mende., Thomas J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, N.C. Population Studies Div. ;Miami Univ., Fla. Dept. of Biochemistry.
Year Published 1976
Report Number EPA/600/J-77/084;
Stock Number PB-278 583
Additional Subjects Pesticides ; Sulfur ; Ribosomes ; Liver ; Metabolism ; Chemical bonds ; Chemical reactions ; Cysteine ; Reprints ; Parathion ; Paraxon ; Metabolites ; Sulfides
Library Call Number Additional Info Location Last
NTIS  PB-278 583 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
In order to investigate the nature of the sulfur that is bound to liver microsomes concurrently with the metabolism of parathion to paraoxon, an isolated rat liver microsomal preparation was labeled with (35S) parathion and the purified product was examined by chemical degradation. Characterization of the degradation products by thin-layer chromatography indicated that the sulfur is bound as a polysulfide or hydrodisulfide formed by the combination of a reactive form of sulfur, released from parathion, with cysteine residues of the microsomal protein. Preliminary investigations revealed similar binding occurs when the microsomal preparation is replaced with purified rabbit cytochrome P-450 plus NADPH-cytochrome P-450 reductase and NADPH. (Copyright (c) 1977 by The Williams & Wilkins Co.)