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Main Title Transfection of cytochrome P450 CDNAS into mammalian cells used in mutation and transformation assays. {microfiche}
Author Langenbach, R. ; Crespi, C. ; Davies, R. ; Rudo, K. ; Smith., P.
CORP Author National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Cellular and Genetic Toxicology Branch. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Gentest Corp., Woburn, MA.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher National Institute of Environmental Health Sciences,
Year Published 1991
Report Number EPA-68-02-4456; EPA/600/D-90/169
Stock Number PB91-132951
Additional Subjects Deoxyribonucleic acids ; Mutagens ; Cells(Biology) ; Mammals ; In vitro analysis ; Carcinogens ; Reprints ; Transfection ; Mutagenicity tests ; Genetic transformation ; Cytochrome P450 ; Cell survival ; Enzymologic gene expression regulation ; Carcinogenicity tests
Library Call Number Additional Info Location Last
NTIS  PB91-132951 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12 p. : ill. ; 28 cm.
The present work demonstrates that cDNAs coding for cytochrome P450 enzymes can be transfected into mammalian cells and expressed. Two different cell systems were used for transfection. The 10T 1/2 cells can be treated with a nontransforming dose of an initiator followed by continuous treatment with a second chemical that requires cytochrome P450 specific metabolism to manifest its promoting activity. By this approach, greater insight into the role of chemical metabolism in the promotion process (and presumably other nongenotoxic effects) can be obtained. Additionally, the role of specific cytochrome P450s in the metabolism of different classes of carcinogens/drugs can be elucidated. A major advantage of having the metabolizing enzymes actually present in the target cell is that effects of chemicals can be studied in long-term, low-dose exposure protocols which will eliminate the acute toxic effects which are associated with many current protocols.
"EPA 600/D-90/169".