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Main Title Effect of Nongenotoxic Environmental Contaminants on Cholesterol and DNA Synthesis in Cultured Primary Rat Hepatocytes.
Author Reddy, T. V. ; Chang, L. W. ; DeAngelo, A. B. ; Pereira, M. A. ; Daniel, F. B. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Cincinnati, OH.
Publisher 1993
Year Published 1993
Report Number EPA/600/J-93/396;
Stock Number PB93-236339
Additional Subjects Liver ; Environmental pollutants ; Cholesterol ; DNA replication ; Rats ; Cultured cells ; Biological models ; Cell division ; Biosynthesis ; Chlorinated hydrocarbons ; Dimethyl sulfoxide ; Epidermal growth factor-urogastrone ; Phenobarbital ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-236339 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
The effects of certain reputedly non-genotoxic agents on cholesterol and DNA synthesis were investigated in cultured primary rat hepatocytes and in liver slices. Hepatocytes in culture were incubated for 48, 60, and 72 hours with one of the following chemicals: chloroform (CHCl3); 1,1,2-trichloroethane (TCE); trichloroacetic acid (TCA); dichloroacetic acid (DCA); monochloroacetic acid (MCA); di(2-ethylhexyl)phthalate (DEHP); mono(ethylhexyl)phthalate (MEHP); phenobarbital (PB); epidermal growth factor (EGF); dimethylsulfoxide (DMSO). All test chemicals stimulated cholesterol and DNA synthesis in the cultures. However, the induction of DNA synthesis in hepatocytes treated with DCA became apparent by sixty hours.