Record Display for the EPA National Library Catalog


Main Title Quantification in Soil of 'Bacillus thuringiensis' var. 'kurstaki' delta-Endotoxin from Transgenic Plants.
Author Palm, C. J. ; Donegan, K. ; Harris, D. ; Seidler, R. J. ;
CORP Author Corvallis Environmental Research Lab., OR. ;ManTech Environmental Technology, Inc., Corvallis, OR.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/344;
Stock Number PB94-210515
Additional Subjects Transgenic plants ; Genetic engineering ; Soil ; Toxins ; Biotechnology ; Pest control ; Plant genetics ; Proteins ; Plant ecology ; Cotton plants ; Biological insect control ; Assaying ; Reprints ; Bacillus thuringiensis kurstaki delta-endotoxin ; Btk toxin ; Bacillus thuringiensis toxin ; Bt toxin
Library Call Number Additional Info Location Last
NTIS  PB94-210515 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Transgenic plants that produce pesticidal proteins have the potential to release these products into the environment when the plants are incorporated into soil. This could result in novel exposure of soil organisms to these pesticidal proteins. There is a lack of knowledge about the fate and persistence of transgenic pesticidal products in the soil. A model system of transgenic cotton, which produces Bacillus thuringiensis kurstaki (delta)-endotoxin (Bt toxin), was used to address this issue. Methods were developed to quantify Btk toxin in soil and soil/plant litter by extraction of the Btk toxin with an aqueous buffer and quantification by ELISA. The highest recovery of Btk toxin from soil was obtained with a high salt, high pH buffer. The method was shown to be useful in tracking over time the persistence of both purified and transgenic Btk toxin in laboratory experiments.