||Phoxocephalid Amphipod Bioassay for Marine Sediment Toxicity.
Swartz, R. C. ;
DeBen, W. A. ;
Jones, J. K. P. ;
Lamberson, J. O. ;
Cole, F. A. ;
||Environmental Research Lab., Narragansett, RI.
Water pollution ;
Animal behavior ;
Particle size ;
||Some EPA libraries have a fiche copy filed under the call number shown.
The relative toxicity of marine sediment can be accurately determined through acute, static bioassays with the phoxocepalid amphipod Repoxynius abronius. Mortality and sublethal effects on emergence from sediment and reburial behavior are determined after ten day exposure in 1-L beakers containing 175 ml of test sediment, 775 ml of seawater (25 ppt, 15 deg C), and 20 amphipods. Response of amphipods to test sediment is compared with response in control sediment collected from the species' natural habitat. Mean survival under control conditions is 95%. With five replicates, the bioassay is 75% certain of detecting statistical significance when mean survival is reduced by 15%. The method can be applied to a great variety of sediment types because of the tolerance of R. abronius to a broad range of sediment grain sizes and levels of organic enrichment.