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Main Title Validation of Protocols for Assessing Early Pregnancy Failure in the Rat: Clomiphene Citrate.
Author Cummings, A. M. ; Perreault, S. D. ; Harris, S. T. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;NSI Technology Services Corp., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/118;
Stock Number PB91-207118
Additional Subjects Spontaneous abortion ; Clomiphene ; Toxicology ; Reproduction(Biology) ; Estrogen antagonists ; Clinical trials ; Animal disease models ; Embryo transfer ; Ovum implantation ; Dose-response relationships ; Corpus luteum ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-207118 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Following the assembly of a battery of protocols for the assessment of maternally-mediated toxicity during early pregnancy, the validation of the battery for its utility in detecting and defining mechanisms of early pregnancy failure is ongoing. The report describes the use of clomiphene citrate (CC), an estrogen agonist/antagonist, in these protocols as part of the validation process. The several protocols involve dosing rats with CC during and evaluation of multiple endpoints following (1) the first 8 days of pregnancy; (2) early pseudopregnancy, accompanied by decidual induction; and (3) the pre- and post implantation intervals of early pregnancy. In addition, the effect of CC on embryo transport rate was assessed. The data suggest a direct effect of CC on the embryo or on its ability to implant as the primary mechanism mediating CC-induced early pregnancy failure. The battery of protocols was thus successful in identifying CC as a reproductive toxicant and in elucidating the causes of the observed early pregnancy failure.