| Main Title |
Method for Measuring Bacterial Resistance to Metals Employing Epifluorescent Microscopy. |
| Author |
Zelibor, J. L. ;
Tamplin, M. ;
Colwell, R. R. ;
|
| CORP Author |
Maryland Univ. at Baltimore.;Environmental Research Lab., Gulf Breeze, FL.;Department of the Interior, Washington, DC. |
| Year Published |
1987 |
| Report Number |
DI-14-08-0001-G-884; EPA/600/J-87/363; |
| Stock Number |
PB88-219803 |
| Additional Subjects |
Bacteria ;
Metals ;
Resistance ;
Bioassay ;
Tissue culture ;
Reprints ;
Metal sensitive strains
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB88-219803 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
15p |
| Abstract |
A direct viable counting method has been developed which can be used to measure resistance of bacteria to metal (DVCMR bio-assay). Results obtained using DVCMR was compared with classical culture methods and proven superior. Evaluation of test strains resistant to arsenic or manganese showed a parabolic curve, whereas sensitive strains demonstrated a logarithmic curve fit. The DVCMR bio-assay method to determine percent resistance (%R) was approximately twice as sensitive for detecting metal-resistant strains and 23 times for metal-sensitive strains, compared with cultural methods. It is concluded that the DVCMR bio-assay may be better suited for use in microbial biogeochemistry, i.e., contamination monitoring and mineral prospecting, than existing cultural methods. |
