Record Display for the EPA National Library Catalog


Main Title Non-Invasive Neurotoxicity Assay Using Larval Medaka.
Author Featherstone, D. ; Drewes, C. D. ; Coats, J. R. ; Bradbury, S. P. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;Iowa State Univ., Ames. ;Iowa Agricultural and Home Economics Experiment Station, Ames.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-94/019;
Stock Number PB94-140621
Additional Subjects Larvae ; Fishes ; Toxicity ; Tests ; Electrophysiology ; Exposure ; Electrodes ; Response ; Phenols ; Reprints ; Medaka ; Neurotoxicity
Library Call Number Additional Info Location Last
NTIS  PB94-140621 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 16p
The authors present a method for non-invasive electrophysiological analysis of rapid escape responses in intact, freely behaving larval medaka (Oryzias latipes) before and after short-term exposure to environmental toxicants. Recordings are obtained as a larval medaka swims in a small chamber of water above pairs of recording electrodes etched onto a printed circuit board. When the chamber is briefly vibrated by a sinusoidal pulse from a speaker attached to the grid, a stereotyped Mauthner cell-initiated escape response is evoked. The following parameters were quantified from recordings: (1) Mauthner axon conduction velocity, (2) delay between Mauthner axon spike and motoneuron spike in the spinal cord, (3) latency from stimulus onset to Mauthner spike, and (4) percent of success (or failure) of Mauthner responses during a series of stimuli. Toxicant-induced changes in these parameters were described following exposure to phenol.