Record Display for the EPA National Library Catalog


Main Title Methodology for Evaluating Potential Human Health Effects of Microorganisms That Degrade Hazardous Wastes.
Author George, S. E. ; Kohan, M. J. ; Walsh, D. B. ; Claxton, L. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/D-87/209;
Stock Number PB87-203063
Additional Subjects Microorganisms ; Biodeterioration ; Hazardous materials ; Public health ; Mutations ; Pseudomonas ; Tests ; Mice ; Metabolism ; Feces ; Monitoring ; Biphenyl ; Chlorine organic compounds ; Antiobiotics ; Intestines ; Dosage ; Exposure ; Statistical analysis ; Counting methods ; Polychlorinated biphenyls ; Biphenyl/chloro
Library Call Number Additional Info Location Last
NTIS  PB87-203063 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 29p
Microorganisms are being developed to environmentally degrade hazardous wastes. Before such organisms are deployed, methods need to be designed to monitor the organisms and waste by-products for potentially adverse human health and environmental effects. Initial work in the authors laboratory involved the study of the biological effects of a mutant microorganisms that biodegrades polychlorinated biphenyl compounds (PCB's). The authors have designed several mouse model systems to examine the ability of these organisms to colonize the intestines of the mouse and compete with the resident intestinal floral. The hazardous waste degrading microorganisms, Pseudomonas aeruginosa, was recoverable on a selective medium in all methods evaluated. The normal intestinal flora was also monitored. Depending on the method utilized, an alteration in one or more of the normal flora was observed in a dose related manner.