The report is an introduction to the basic techniques involved in teratology testing. General topics covered include the handling of animals, compound preparation and administration, dosage calculations, and coding techniques. Teratology techniques include fetal fixation, alizarin staining, and the examination of fixed and stained fetuses for soft tissue and skeletal anomalies. Suitable protocol sheets covering data obtained before sacrifice, after sacrifice, and subsequent fetal examinations are included. A glossary of the most common malformations and their description is present. The outlines of a teratology experiment with Vitamin A is included as a suggested training exercise. The above experiment and the techniques described in the manual may be used to train laboratory personnel in the techniques and designs of teratology experiments.

"EPA/650-1-73-001." "September 1974."