||Physiological Properties and Substrate Specificity of a Pentachlorophenol-Degrading 'Pseudomonas' Species.
Resnick, S. M. ;
Chapman, P. J. ;
||Technical Resources, Inc., Gulf Breeze, FL.;Environmental Research Lab., Gulf Breeze, FL.
Soil pollution ;
Water pollution ;
Wood preservatives ;
Pseudomonas vesicularis ;
Substrate specificity ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Isolation of a bacterial strain capable of utilizing pentachlorophenol (PCP) as sole source of carbon and energy for growth was facilitated by using enrichment cultures inoculated with contaminated soil from a lumber treatment waste site. This isolate was provisionally characterized as a strain of Pseudomonas vesicularis (strain SR3). Evidence for the ability of the pure culture to mineralize PCP was based on measurements of complete loss of PCP, stoichiometry of chloride release (5 equivalents of chloride per mole PCP), and formation of biomass in proportion to concentration of PCP. Washed cells of PCP-induced SR3 showed elevated rates of oxygen consumption in the presence of PCP and with a range of tetra-, tri-, di and mono-substituted chlorphenols.