Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Biodegradation of Creosote and Pentachlorophenol in Contaminated Groundwater: Chemical and Biological Assessment.
Author Mueller, J. G. ; Middaugh, D. P. ; Lantz, S. E. ; Chapman., P. J. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Southern BioProducts, Inc., Pendleton, SC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/328 ;CONTRIB-728;
Stock Number PB92-129659
Additional Subjects Biodeterioration ; Water pollution control ; Biological treatment ; Hazardous materials ; Ground water ; Creosote ; Phenol/pentachloro ; Waste disposal ; Remedial action ; Superfund ; Chemical spills ; Gas chromatography ; Chemical analysis ; Chemical reactions ; Biological effects ; Toxicity ; Wood preservatives ; Microorganisms ; Biotechnology ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-129659 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 08/28/1992
Collation 11p
Shake flask studies examined the rate and extent of biodegradation of pentachlorophenol (PCP) and 42 components of coal-tar creosote present in contaminated groundwater recovered from the American Creosote Works Superfund site, Pensacola, Fla. The ability of indigenous soil microorganisms to remove these contaminants from aqueous solutions was determined by gas chromatographic analysis of organic extracts of biotreated groundwater. Changes in potential environmental and human health hazards associated with the biodegradation of the material were determined at intervals by Microtox assays and fish toxicity and teratogenicity tests. After 14 days of incubation at 30 C, indigenous microorganisms effectively removed 100, 99, 94, 88, and 87% of measured phenolic and lower-molecular-weight polycyclic aromatic hydrocarbons (PAHs) and S-heterocyclic, N-heterocyclic, and O-heterocyclic constituents of creosote, respectively. However, only 53% of the higher-molecular-weight PAHs were degraded; PCP was not removed. Despite the removal of a majority of the organic contaminants through biotreatment, only a slight decrease in the toxicity and teratogenicity of biotreated groundwater was observed. Data suggest that toxicity and teratogenicity are associated with compounds difficult to treat biologically and that one may not necessarily rely on indigenous microorganisms to effectively remove these compounds in a reasonable time span; to this end, alternative or supplemental approaches may be necessary. Similar measures of the toxicity and teratogenicity of treated material may offer a simple, yet important, guide to bioremediation effectiveness. (Copyright (c) 1991, American Society for Microbiology.)