| Main Title |
Identification of Organic N-Chloramines In vitro in Stomach Fluid from the Rat After Chlorination. |
| Author |
Scully, F. E. ;
Mazina, K. E. ;
Ringhand, H. P. ;
Chess, E. K. ;
Campbell., J. A. ;
|
| CORP Author |
Old Dominion Univ., Norfolk, VA. Dept. of Chemical Sciences. ;Battelle Pacific Northwest Labs., Richland, WA. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Health Effects Research Lab., Cincinnati, OH. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA/600/J-90/510; |
| Stock Number |
PB91-199968 |
| Additional Subjects |
Chlorination ;
Stomach contents ;
Chloramines ;
Rats ;
In vitro analysis ;
Temperature ;
pH ;
Isotopes ;
Amino acids ;
High performance liquid chromatography ;
Mass spectrometry ;
Ions ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-199968 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
When (36)Cl-N-cholorpiperidine (NCP) (300 mg/L as Cl2) was mixed with stomach fluid from laboratory rats (fasted either 24 or 48 hr) and incubated in the dark for 30 min at 37 C and at higher pH values, approximately one-third was reduced to chloride and between 7% and 63% of the radiolabel was found to chromatograph in a manner distinctly different from (36)Cl-chloride of (36)Cl-NCP. The new fraction is referred to as the (36) Cl-chloroorganic fraction. The remainder of the radiolabel was associated with (36)Cl-NCP. Varying concentrations of (36)Cl-NCP (3-200 mg/L as Cl2) have been incubated for 30 min at 37 C with stomach fluid at varying pH on the product distribution. At low pH values a threshold concentration of approximately 100 mg/L active chlorine was measured below which all chloramine was reduced to (36)Cl-chloride. As the pH and concentration of (36)Cl-NCP were increased, the percentage of chloramine reduced to chloride decreased. Using concentrations between 110 and 120 mg/L (Cl2) 7% and 4% of the label was associated with the (36)Cl-chloroorganic fraction at pH 7.1 and at pH 2.2, respectively. |
