Record Display for the EPA National Library Catalog


Main Title Assay of Chicken Brain Neurotoxic Esterase Activity Using Leptophosoxon as the Selective Neurotoxic Inhibitor.
Author Soliman, Salah A. ; Curley, August ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Environmental Toxicology Div.
Year Published 1981
Report Number EPA-600/J-81-235;
Stock Number PB82-127598
Additional Subjects Esterases ; Toxicology ; Brain ; Inhibitors ; Chickens ; Bioassay ; Reprints ; Neurotoxins
Library Call Number Additional Info Location Last
NTIS  PB82-127598 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
Hen brain microsomal preparation has phenyl valeratehydrolyzing activity associated with neurotoxic esterase activity. Part of that activity is due to paraoxon-insensitive esterases and a sub-part of this is sensitive to neurotoxic organophosphates, i.e., mipafox and leptophosoxon. This neurotoxic agent sensitive esterase activity is referred to as neurotic esterase (NTE). Because of the commercial unavailability and high toxicity of mipafox, which is usually used as the selective inhibitor for assaying NTE, leptophosoxon was used as an alternative to mipafox. Results indicated that the NTE fraction of hen brain microsomal PV-hydrolyzing activity is the same target for either mipafox or leptophosoxon. The inhibitory effect of leptophosoxon against that fraction was much higher than that of mipafox. The availability of leptophos/leptophosoxon makes this assay very useful for screening organophosphorus esters for neurotoxic effects.