Record Display for the EPA National Library Catalog


Main Title Hydrogenase-Linked Gene in 'Methanobacterium thermoautotrophicum' Strain DeltaH Encodes a Polyferredoxin.
Author Reeve, J. N. ; Beckler, G. S. ; Cram, D. S. ; Hamilton, P. T. ; Brown, J. W. ;
CORP Author Ohio State Univ., Columbus. Dept. of Microbiology. ;Massachusetts Inst. of Tech., Cambridge. Dept. of Chemistry. ;Harvard Medical School, Boston, MA. Dept. of Biological Chemistry and Molecular Pharmacology.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1989
Year Published 1989
Report Number EPA-R-812774; EPA/600/J-92/242;
Stock Number PB92-206234
Additional Subjects Methanogenic bacteria ; Hydrogenase ; Ferredoxins ; Bacterial genes ; Molecular cloning ; Open reading frames ; Molecular sequence data ; Nucleic acid sequence homology ; Reprints ; Methanobacterium thermoautotrophicum
Library Call Number Additional Info Location Last
NTIS  PB92-206234 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
The genes, mvhDGA which encode the subunit polypeptides of methyl viologen reducing hydrogenase in Methanobacterium thermoautotrophicum strain DeltaH have been cloned and sequenced. These genes, together with a fourth open reading frame designated mvhB, are tightly linked and appear to form an operon which is transcribed starting 42bp upstream of mvhD. The organization and sequences of the mvhG and mvhA genes indicate a common evolutionary ancestry with genes encoding the small and large subunits of hydrogenases in eubacterial species. The product of the mvhB gene is predicted to contain six tandomly-repeated, bacterial ferredoxin-like domains and is therefore predicted to be a poly-ferredoxin which could contain as many as 48 iron atoms in 12 Fe4S4 clusters.