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Main Title N-Nitrosodiethylamine-Induced Hepatocarcinogenesis in Estuarine Sheepshead Minnow ('Cyprinodon variegatus'): Neoplasms and Related Lesions Compared with Mammalian Lesions.
Author Couch, J. A. ; Courtney, L. A. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL.
Year Published 1987
Report Number EPA/600/J-87/188;
Stock Number PB88-161799
Additional Subjects Reprints ; Sheepshead minnows ; Cyprinodon variegatus ; DENA ; Nitrosodiethylamine ; Hepato carcinogenesis
Library Call Number Additional Info Location Last
NTIS  PB88-161799 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 27p
Groups of estuarine sheepshead minnows (Cyprinodon variegatus) were exposed to 50-60 mg/l N-nitrosodiethylamine (DENA) for five to six weeks. Exposure was stopped and the fish were then transferred to clean, flowing seawater. Induced liver lesions were studied in periodic samples of fish taken during the next 67 weeks of holding. Most of these lesions were compared to their counterpart lesions in the rat. Certain lesions such as hepatocellular carcinomas, cholangiolar carcinomas, spongiosis hepatis (SH), and cholangiofibrosis in the fish have apparent similar cellular origins and morphogenesis to those Lesions in rats, and perhaps in other mammals. SH in the sheepshead minnow apparently arises from perisinusoidal cells and may be a neoplasm of the cell type. The general similarity of response to DENA in sheepshead minnows and rats suggests that the fish has promise as an assay subject for identifying some hepatocarcinogens, and as a sentinel organism for detecting hepatocarcinogens in contaminated coastal waters.