Record Display for the EPA National Library Catalog
RECORD NUMBER: 2 OF 11| Main Title | Autographa Californica Nuclear Polyhedrosis Virus Efficiently Enters but Does Not Replicate in Poikilothermic Vertebrate Cells. | |||||||||||
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| Author | Brusca, J. ; Summers, M. ; Couch, J. ; Courtney, L. ; | |||||||||||
| CORP Author | Texas A and M Univ., College Station. ;Texas Agricultural Experiment Station, College Station.;Environmental Research Lab., Gulf Breeze, FL. | |||||||||||
| Year Published | 1986 | |||||||||||
| Report Number | EPA-R-810461-01-1; EPA/600/J-86/428; | |||||||||||
| Stock Number | PB88-149174 | |||||||||||
| Additional Subjects | Virology ; Infectious ; diseases ; Poikilothermic ; Vertebrates ; Cells ; Cytology ; Hybridization ; Replicating ; Viruses ; Nucleic acids ; Electron microscopy ; Reprints ; Autographa californica nuclear polyhedrosis ; virus ; Viral proteins | |||||||||||
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| Collation | 18p | |||||||||||
| Abstract | The host range of the insect virus Autographa californica nuclear polyhedrosis virus (AcMNPV) was examined. AcMNPV could not initiate a productive infection in frog, turtle, trout, or moth cell lines. After exposure to AcMNPV, neither viral DNA nor RNA synthesis could be detected in these cell lines when assayed by dot-blot hybridizations. Entry of viral DNA to the nucleus, however, was as efficient in the nonpermissive cell lines as it was in a permissive insect cell line. Electron microscopy revealed numerous AcMNPV nucleocapsids in the cytoplasm of the nonpermissive cell lines is therefore at a stage subsequent to viral entry to the nucleus. (Copyright (c) 1986, Intervirology.) |