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Main Title Autographa Californica Nuclear Polyhedrosis Virus Efficiently Enters but Does Not Replicate in Poikilothermic Vertebrate Cells.
Author Brusca, J. ; Summers, M. ; Couch, J. ; Courtney, L. ;
CORP Author Texas A and M Univ., College Station. ;Texas Agricultural Experiment Station, College Station.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1986
Report Number EPA-R-810461-01-1; EPA/600/J-86/428;
Stock Number PB88-149174
Additional Subjects Virology ; Infectious ; diseases ; Poikilothermic ; Vertebrates ; Cells ; Cytology ; Hybridization ; Replicating ; Viruses ; Nucleic acids ; Electron microscopy ; Reprints ; Autographa californica nuclear polyhedrosis ; virus ; Viral proteins
Library Call Number Additional Info Location Last
NTIS  PB88-149174 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 18p
The host range of the insect virus Autographa californica nuclear polyhedrosis virus (AcMNPV) was examined. AcMNPV could not initiate a productive infection in frog, turtle, trout, or moth cell lines. After exposure to AcMNPV, neither viral DNA nor RNA synthesis could be detected in these cell lines when assayed by dot-blot hybridizations. Entry of viral DNA to the nucleus, however, was as efficient in the nonpermissive cell lines as it was in a permissive insect cell line. Electron microscopy revealed numerous AcMNPV nucleocapsids in the cytoplasm of the nonpermissive cell lines is therefore at a stage subsequent to viral entry to the nucleus. (Copyright (c) 1986, Intervirology.)