Record Display for the EPA National Library Catalog


Main Title Cytogenetic Characterization of the L5178Y TK+/-3.7.2C Mouse Lymphoma Cell Line.
Author Sawyer, J. ; Moore, M. M. ; Clive, D. ; Hozier, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Florida Inst. of Tech., Melbourne. ;Burroughs Wellcome Co., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/408;
Stock Number PB86-212628
Additional Subjects Mice ; Reprints ; Cell lines ; Cytogenetics ; Mouse lymphoma cell lines
Library Call Number Additional Info Location Last
NTIS  PB86-212628 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
The cytogenetic characterization of the L5178Y TK+/-3.7.2C mouse lymphoma cell line was carried out, utilizing G-banded metaphase chromosomes, to provide a karyotypic basis for the precise delineation of induced rearrangements in TK-/- mutants. Band-pattern measurements were used to construct ideograms which represent the position, number, size and staining intensity of the chromosome bands. The TK+/- 3.7.2C cell line has been shown to provide quantitation of forward mutations induced at the autosomal thymidine kinase (TK) locus in the cell line. Chromosome analysis of the TK+/-3.7.2C cell line and derived TK-/- mutants has become important in demonstrating that the TK+/- -> TK-/- assay may detect and distinguish between chromosomal events and smaller, perhaps point-mutation, events in mutant colonies.