Record Display for the EPA National Library Catalog


Main Title Mutation and Enhanced Virus Transformation of Cultured Hamster Cells by Exposure to Gaseous Ethylene Oxide.
Author Hatch, G. G. ; Conklin, P. M. ; Christensen, C. C. ; Anderson, T. M. ; Langenbach, R. ;
CORP Author Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Year Published 1986
Report Number EPA-68-02-2566; EPA/600/J-86/096;
Stock Number PB86-209830
Additional Subjects Toxicology ; Genes ; Viruses ; Mutations ; In vitro analysis ; Cells(Biology) ; Reprints ; Ethylene oxide
Library Call Number Additional Info Location Last
NTIS  PB86-209830 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Ethylene oxide is a classical mutagen and a carcinogen based on evidence from studies in experimental animals. It is widely distributed in industrial, research, hospital, and food environments. In an effort to explore the use of newly developed methods for exposing mammalian cells to gaseous or volatile mutagens/carcinogens, Chinese hamster V79 cells were treated for 2 hr with gaseous ethylene oxide, in sealed treatment chambers and assayed for survival and mutagenic response by analysis of induced resistance to 6-thioguanine or ouabain. Significant numbers of mutants were produced at both genetic markers by 1,250-7,500 ppm ethylene oxide. Similarly, primary Syrian hamster embryo cells were treated for 2 or 20 hr with gaseous ethylene oxide in sealed treatment chambers and subsequently assayed for survival and increased sensitivity to SA7 virus transformation. Treatment concentrations extended from toxic to several nontoxic concentrations.