Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Determination of Trace Level Hydrocarbons in Marine Biota.
Author Chesler, S. N. ; Gump, B. H. ; Hertz, H. S. ; May, W. E. ; Wise., S. A. ;
CORP Author National Bureau of Standards, Washington, D.C.;Environmental Protection Agency, Washington, D.C.;Bureau of Land Management, Washington, D.C.
Year Published 1978
Stock Number PB-281 922
Additional Subjects Hydrocarbons ; Trace elements ; Petroleum products ; Water pollution ; Gas chromatography ; Chemical analysis ; Marine atmospheres ; Aquatic animals ; Water pollution detection ; Water pollution effects(Animals) ; Baseline measurements ; Reprints
Library Call Number Additional Info Location Last
NTIS  PB-281 922 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 6p
A method is described for the analysis of petroleum hydrocarbons in marine biota. The method is suitable for the determination of the low hydrocarbon levels encountered in pollution baseline studies as well as the higher levels found in organisms exposed to petroleum. The procedure utilizes dynamic headspace sampling of an aqueous caustic tissue homogenate to extract and collect volatile organic components. Interfering polar biogenic components are removed by normal-phase high-performance liquid chromatography(HPLC). Quantitation and identification of the individual compounds are accomplished using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The non-volatile components remaining in the homogenate after headspace sampling are solvent extracted and analyzed by reversed-phase liquid chromatography.