| Main Title |
Determination of Trace Level Hydrocarbons in Marine Biota. |
| Author |
Chesler, S. N. ;
Gump, B. H. ;
Hertz, H. S. ;
May, W. E. ;
Wise., S. A. ;
|
| CORP Author |
National Bureau of Standards, Washington, D.C.;Environmental Protection Agency, Washington, D.C.;Bureau of Land Management, Washington, D.C. |
| Year Published |
1978 |
| Stock Number |
PB-281 922 |
| Additional Subjects |
Hydrocarbons ;
Trace elements ;
Petroleum products ;
Water pollution ;
Gas chromatography ;
Chemical analysis ;
Marine atmospheres ;
Aquatic animals ;
Water pollution detection ;
Water pollution effects(Animals) ;
Baseline measurements ;
Reprints
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB-281 922 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
A method is described for the analysis of petroleum hydrocarbons in marine biota. The method is suitable for the determination of the low hydrocarbon levels encountered in pollution baseline studies as well as the higher levels found in organisms exposed to petroleum. The procedure utilizes dynamic headspace sampling of an aqueous caustic tissue homogenate to extract and collect volatile organic components. Interfering polar biogenic components are removed by normal-phase high-performance liquid chromatography(HPLC). Quantitation and identification of the individual compounds are accomplished using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The non-volatile components remaining in the homogenate after headspace sampling are solvent extracted and analyzed by reversed-phase liquid chromatography. |
