Record Display for the EPA National Library Catalog


Main Title Evidence for a Novel Pathway in the Degradation of Fluorene by 'Pseudomonas' sp. Strain F274.
Author Grifoll, M. ; Selifonov, S. A. ; Chapman, P. J. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Barcelona Univ. (Spain). Dept. of Microbiology. ;University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/439;
Stock Number PB95-112207
Additional Subjects Fluorene ; Catabolism ; Soil pollution ; Reprints ; Pseudomonas ; Biodegradation ; Soil microbiology ; Polycyclic aromatic hydrocarbons ; Toxic substances ; Metabolites ; Pseudomonas sp strain F274 ; Microbial degradation
Library Call Number Additional Info Location Last
NTIS  PB95-112207 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
A fluorene-utilizing microorganism, identified as a species of Pseudomonas, was isolated from soil severely contaminated from creosote use and was shown to accumulate six major metabolites from fluorene in washed-cell incubations. Five of these products were identified as 9-fluorenol, 9-fluorenone, (+)-1,1a-dihydroxy-1-hydro-9-fluorenone, 8-hydroxy-3,4-benzocoumarin, and phthalic acid. This last compound was also identified in growing cultures supported by fluorene. Fluorene assimulation into cell biomass was estimated to be approximately 50%. The structures of accumulated products indicate that a previously undescribed pathway of fluorene catabolism is employed by Pseudomonas sp. strain F274. On the basis of these findings, a pathway of fluorene degradation is proposed to account for its conversion to intermediary metabolites. A range of compounds with structures similar to that of fluorene was acted on by fluorene-grown cells to give products consistent with the initial reactions proposed.