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Main Title Characterization and Nucleotide Sequence Determination of a Repeat Element Isolated from a 2,4,5-T Degrading Strain of 'Pseudomonas cepacia'.
Author Tomasek, P. H. ; Frantz, B. ; Sangodkar, U. M. X. ; Haugland, R. A. ; Chakrabarty, A. M. ;
CORP Author Abraham Lincoln School of Medicine, Chicago, IL.;Environmental Research Lab., Gulf Breeze, FL.;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number PHS-ES-04050; EPA/600/J-89/171;
Stock Number PB90-129560
Additional Subjects Genetics ; Deoxyribonucleic acids ; Binding ; Reprints ; Pseudomonas cepacia ; Nucleic acid repetitive sequences ; Trichlorophenoxyacetic acid ; Plasmids ; Molecular cloning ; DNA insertion elements ; Nucleic acid sequence homology
Library Call Number Additional Info Location Last
NTIS  PB90-129560 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
Pseudomonas cepacia strain AC1100, capable of growth on 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), was mutated to the 2,4,5-T(sup -) strain PT88 by a ColE1::Tn5 chromosomal insertion. Using cloned DNA from the region flanking the insertion, a 1477-bp sequence (designated RS1100) was identified which was repeated several times on the wild-type chromosome and was also present on AC1100 plasmid DNA. Various chromosomal fragments containing this sequence were cloned and their nucleotide sequence was determined. Examination of TS1100 revealed the presence of 38-39-bp terminal inverted repeats immediately flanked by 8-bp direct repeats. The translated sequence of the single large open reading frame of RS1100 showed structural similarity to the phage Mu transposes and other DNA-binding proteins. (Copyright (c) 1989 Elsevier Science Publishers B.V.)