||Evaluation of m-T7 Agar as a Fecal Coliform Medium
LeChevallier, M. W. ;
Jakanoski, P. E. ;
Camper, A. K. ;
McFeters, G. A. ;
||Montana State Univ., Bozeman. Dept. of Microbiology.;Environmental Protection Agency, Cincinnati, OH. Drinking Water Research Div.
Coliform bacteria ;
Culture media ;
||Some EPA libraries have a fiche copy filed under the call number shown.
The m-T/7 agar, designed to improve recoveries of injured total coliforms, was evaluated for its effectiveness as a fecal coliform medium. The time and temperature of preincubation were found to be crucial to the optimal recovery of fetal coliforms. Isolation rates for fecal coliforms on m-T7 agar from sewage effluents were the highest when plates were preincubated at 37C for 8 h before transfer to 44.5C for 12 h. The medium was found to produce consistently higher fecal coliform counts than all the other methods tested. Recoveries were 3.1 times greater than the standard m-FC method and 1.7 times greater than the two-layer enrichment, temperature acclimation procedure. Verification rates for fecal coliforms isolated on m-T7 agar averaged 89.0%, whereas verification rates for m-FC agar averaged only 82.8%. (Copyright (c) 1984, American Society for Microbiology.)