Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Effect of Exogenous Glutathione, Glutathione Reductase, Chlorine Dioxide, and Chlorite on Osmotic Fragility of Rat Blood In vitro.
Author Abdel-Rahman, M. S. ; Couri, D. ; Bull, R. J. ;
CORP Author New Jersey Medical School, Newark. Dept. of Pharmacology. ;Ohio State Univ., Columbus. Coll. of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA-R-807473; EPA/600/J-84/174;
Stock Number PB85-146744
Additional Subjects Chlorine dioxide ; Chlorite ; Chlorate ; Toxicology ; In vivo analysis ; Rats ; Blood ; In vitro analysis ; Reprints ; Glutathione
Library Call Number Additional Info Location Last
NTIS  PB85-146744 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 9p
Chlorine dioxide (ClO2), chlorite (ClO2(-1)), and chlorate (ClO3(-1)) in drinking water decreased blood glutathione and RBC osmotic fragility in vivo. The osmotic fragility and glutathione content were also studied in rat blood treated with ClO2, ClO2(-1), ClO3(-1) in vitro. RBC hemolysis was decreased in rat blood after 30, 60, and 120 minutes by all treatments. The glutathione content expressed as percentage of controls was decreased with incubation time. When ClO2 was added with reduced glutathione (GSH) to the blood, no effect on hemolysis was observed compared to control or to GSH alone at 2 hours, but decreased hemolysis was observed with ClO2 treatment alone. Addition of NADPH alone prevented ClO2 and ClO2(-1) and ClO3(-1) from exhibiting hemolysis resistance, while glutathione reductase (GR) and its cofactor (NADPH) increased hemolysis about 1.5-2 fold. Removing GR only resulted in increased resistance to hemolysis with ClO2 or ClO2(-1). The formation of disulfide bonds between sulfhydryl groups in erythrocytic membranes and hemoglobin, causing precipitation of hemoglobin (yielding apparent resistance to hemolysis) can account for the difference between the hemolysis before and after the addition of GR.