Main Title |
Level 1 Bioassay Sensitivity. |
Author |
Brusick, D. J. ;
Young, R. R. ;
|
CORP Author |
Litton Bionetics, Inc., Kensington, MD.;Industrial Environmental Research Lab., Research Triangle Park, NC. |
Year Published |
1981 |
Report Number |
EPA-58-02-2681; EPA-600/7-81-135; |
Stock Number |
PB82-221201 |
Additional Subjects |
Environmental surveys ;
Bioassay ;
In vitro analysis ;
In vivo analysis ;
Assessments ;
Mutagens ;
Samples ;
Toxicity ;
Tables(Data) ;
Concentration(Composition) ;
Industrial hygiene ;
Toxic substances
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB82-221201 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
59p |
Abstract |
The report, based on a survey of published literature, establishes a set of sensitivity estimates for the Level 1 environmental assessment (EA) bioassays recommended by EPA's IERL-RTP. The estimates permit comparison of bioassay sensitivity for detecting toxicity or mutagenicity of pure chemicals and also estimation of the concentrations of toxic materials that could be in a mixture that registers negative in a particular test. Three tests--Ames Salmonella/microsome mutagenesis assay, in vitro rodent cell (CHO) clonal toxicity assay, and in vivo rodent toxicity assay--have substantial published data bases using study designs similar to those used in Level 1 EA tests. The report summarizes the sensitivity limits for detecting toxicity or mutagenicity for a variety of pure compounds with these three tests. Methods developed to assess these data will be applicable to other Level 1 tests such as the rabbit alveolar macrophage (RAM) assay and the aquatic and other ecological assays. However, these evaluations await the development of a sufficient data base with a wide variety of pure compounds. Two of the reviewed bioassays (Ames and CHO) were found to approach the sensitivity required of the Level 1 chemical analytical procedures. |