Record Display for the EPA National Library Catalog


Main Title Use of short-term bioassays to evaluate environmental impact of land treatment of hazardous industrial waste
Author Brown, Kenneth Warren ; Donnelly, K. C. ; Thomas, J. C.
Other Authors
Author Title of a Work
Donnelly, K. C.
Thomas, J. C.
CORP Author Texas Agricultural Experiment Station, College Station.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Publisher U.S. Environmental Protection Agency, Robert S. Kerr Environmental Research Laboratory;
Year Published 1984
Report Number EPA-600/2-84-135; EPA-R-807701; PB84232560
Stock Number PB84-232560
OCLC Number 16209388
Subjects Factory and trade waste--Environmental aspects--United States ; Waste disposal in the ground--Environmental aspects--United States
Additional Subjects Environmental impacts ; Hazardous materials ; Solid waste disposal ; Bioassay ; Industrial wastes ; Soil analysis ; Mutagens ; Chemical analysis ; Toxicity ; Extraction ; Chromatographic analysis ; Land disposal ; Toxic substances ; High pressure liquid chromatography
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EMBD  EPA/600/2-84/135 NRMRL/GWERD Library/Ada,OK 05/01/1992
NTIS  PB84-232560 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation xxvii, 357 p. : ill. ; 28cm.
A four phase study was conducted to evaluate utility of short-term bioassays in monitoring environmental impact of land treatment of hazardous waste. During phase one, three microbial bioassays were conducted to define chronic toxic potential of each waste selected for study. Acid, base, and neutral fractions of each of three wastes studied induced genetic damage in at least two of the three bioassays. Phase two was conducted to evaluate efficiencies of blender and soxhlet extraction procedures, as well as potential interactions between known mutagens and soil components. Results indicate that there was no appreciable difference in mutagenicity of the extract using either procedure. Using the blender procedure extraction efficiency for pure compounds added to soil averaged greater than 85%, as measured by High Pressure Liquid Chromatography. Phase three consisted of a greenhouse study in which each of three wastes was applied to two soils. Results from chemical analyses indicate that waste constituents were degraded in soil during a 360 or 340 day interval. Increased mutagenic activity was exhibited in some soil and water extracts during this same interval. When compared on an equivalent volume basis, however, mutagenic potential of waste-amended soils was reduced over time and, in some cases, was reduced to a non-mutagenic level. Wood-preserving bottom sediment was applied to barrel-sized lysimeters in the final project phase to compare results of soil-core and soil-pore liquid monitoring. Different types of compounds were detected in soil-core and soil pore liquid samples.
"Sept. 1984." "EPA-600/2-84-135." "PB84-232560."