Record Display for the EPA National Library Catalog


Main Title The dynamics of an estuary as a natural ecosystem, II /
Author Vernberg, F. John, ; Koch, Robert B. ; Kitchens, W. ; McKellar, H. ; Summers, K. ; Bonnell, R.
Other Authors
Author Title of a Work
Kitchens, Wiley M.,
McKellar, H. N.
Summers, K.,
Bonnell, R.,
Vernberg, F. John,
CORP Author Mississippi State Univ., Mississippi State. Dept. of Biochemistry.;Environmental Research Lab., Gulf Breeze, FL.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Environmental Research Laboratory,
Year Published 1978
Report Number EPA-600/3-78-092; EPA-R-804407-01
Stock Number PB-289 969
OCLC Number 08081667
Subjects Estuarine ecology--South Carolina ; Estuarine ecology--Mathematical models ; Salt marsh ecology--South Carolina ; Salt marsh ecology--Mathematical models ; Tidemarsh ecology--South Carolina ; Tidemarsh ecology--Mathematical models
Additional Subjects Pesticides ; Antibodies ; Development ; Chlorine organic compounds ; Inhibition ; Recommendations ; Rabbits ; Chickens ; Laboratory animals ; Antigen antibody reactions ; Enzymes ; Insecticides ; Chlorine organic compounds ; Kelevan ; Adenosine triphosphatase ; Kepone ; Immune serums ; Methenocyclobutapentalene levulinic acid/decachlorooctahydro-hydroxy-(ethyl-ester) ; Cyclobutapentalenone/decachlorocathydro ; North Inlet Estuary ; Ecology ; Water pollution ; Mathematical models ; Aquatic plants ; Aquatic animals ; Plankton ; Intertidal zone ; Marine microorganisms ; Invertebrates ; Food chains ; Primary biological productivity ; Fishes ; Birds ; Respiration ; Biomass ; Nutrients ; Computer programming ; South Carolina ; Spartina alterniflora ; Salt marshes ; Baseline studies
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD  EPA 600-3-78-092 c.1 Headquarters Library/Washington,DC 03/24/2014
EJDD  EPA 600/3-78-092 Env Science Center Library/Ft Meade,MD 03/31/1995
EKCD  EPA-600/3-78-092 CEMM/GEMMD Library/Gulf Breeze,FL 07/21/2015
ELBD ARCHIVE EPA 600-3-78-092 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ERAD  EPA 600/3-78-092 Region 9 Library/San Francisco,CA 10/22/2012
ESAD  EPA 600-3-78-092 Region 10 Library/Seattle,WA 08/23/2017
NTIS  PB-289 963 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
NTIS  PB-289 969 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation viii, 76 pages : illustrations ; 28 cm.
The report describes development of an antibody to an organochlorine pesticide to be used in studies related to its inhibition of the ATPase system. Kelevan, the condensation product of ethyl levulinate and Kepone, was successfully conjugated to bovine serum albumin (BSA), fibrinogen (BF), and gamma globulin (BGG). Rabbits and chickens preimmunized with BSA and then immunized with BSA-Kelevan produced antibodies to both the hapten, Kelevan, and the carrier protein BSA. Antiserum to Kelevan protected ATPase activity against Kepone and its derivatives. The titer of antibody to Kelevan was critical since antiserum with only trace amounts of Kelevan antibody failed to protect the ATPase activity against Kepone Inhibition. Antibody was concentrated by Na2S04 fractional precipitation of the antiserum and obtained in pure form by affinity chromatography using BGG-Kel covalently linked to Sepharose 4B. Pure antibody was obtained from untreated blood serum or plasma with no prior pretreatment or fractionation using the BGG-Kel affinity column. Complete protection of mitochondrial mg2+ATPase activity from in vitro inhibition of Kepone was obtained using a 1.2 mg quantity of na2S04 fractionated antibody and only 120 micrograms of pure antibody. Reversal of ATPase inhibition was readily obtained by addition of antibody prior to addition of substrate to the reaction mixture.
"Belle W. Baruch Institute for Marine Biology and Coastal Research, University of South Carolina." "October 1978." Microfiche Includes bibliographical references (pages 72-76).